Mike Riley announced after Tuesday's practice that cornerback Jonathan Rose is no longer on the team.
"Violation of team rules," the Husker head coach said.
Riley had said on Friday that there was one Nebraska player no longer on the team but wasn't ready to name him publicly at that point.
Rose was suspended twice prior this season, missing the opener, then missing the Purdue game due to off-the-field issues.
The senior played in nine games and started five for Nebraska this season, emerging as one of the Huskers' top three corners, along with Joshua Kalu and Chris Jones.
Rose had 42 tackles, seven pass breakups, and one interception this year, coming against Michigan State.
The 6-foot-1, 195-pound native of Leeds, Alabama, arrived to Nebraska in 2012 after being dismissed at Auburn for "personal reasons" after one season.
Rose hadn't started any games at Nebraska prior to this year, but said in early September he was eager to make the most of this final season.
“You know this is your last trip. You don’t want to take any game for granted. That’s how I feel right now," he said. "It’s sad to say, but I’m counting my games. I’m trying to take advantage of every game, every opportunity I get on the field."
Defensive coordinator Mark Banker said Trai Mosley and Boaz Joseph are options to fill that open space, as well senior Daniel Davie, though Davie hasn't been able to practice much yet this bowl season due to his class schedule.
In other news, Riley said redshirt freshman defensive tackle Mick Stoltenberg suffered a knee injury that will require surgery.
"We're going to do it after the bowl game so that he can go to the bowl game as comfortable as he can be right now. That's really unfortunate for us and for Mick, but he'll be ready to go for next season.